Host: The Society of Chemical Engineers, Japan
Name : IChES (International Chemical Engineering Symposia) 2022
Date : March 16, 2022 - March 18, 2022
Pages 9-12
Multi-column chromatography (periodic counter-current chromatography, PCCC) with protein A affinity resins is used as an efficient monoclonal antibody (mAb) continuous capture process. However, the evaluation of the efficiency of PCCC process compared with repeated batch (or cyclic) operation (RBO) is still not standardized. In this study, we proposed two criteria for evaluating the capture process efficiency, productivity P and buffer consumption per load amount, BC. These criteria were calculated for 2 column, 3 column and 4 column-PCCC and for RBO. For PCCC, P was proportional to mAb concentration C0 and inversely proportional to the number of columns, nC since the loaded amount of mAb ML was fixed for the calculation. Bc did not depend on C0 or nC as ML was the same. For RBO, BC was larger by 20% at residence time (RT) = 4 minutes, and by 50% at RT = 2 minutes whereas P was similar to or slightly lower than that for 2C-PCCC at the same RT (4 minutes).
