Development of Cell Chip System for Cytological Analysis and Diagnosis

Abstract

Microchip technologies are expected to perform high-throughput analysis of the function of single-cells. In previous our study, we already developed a single-cell microarray chip for high-throughput screening and analysis of antigen-specific single B-cells. Using the technology of cell microarray chip, we have developed a new system for the high-sensitive and rapid diagnosis of parasitosis. The microarray chip is made from polystyrene with over 10,000 microchambers, which can accommodate 100 erythrocytes. As the result, over 1,000,000 erythrocytes were dispersed on a microarray. We could detect parasites as low as 0.0001% with spreading of human erythrocytes on the microarray followed within 15 min.