Plasmonic-chip Enhanced Fluorescence Biosensor in the Back Illumination System

Abstract

The plasmonic chip which has wavelength-size grating pattern coated with a thin silver or gold film is applied to a sensitive biosensor because it can provide the enhanced fluorescence excited by the surface plasmon field. We have already reported on the sensitive immunodetection with the plasmonic sensor chip under the illumination system from top panel of a plasmonic chip. In this study, the structure of a biosensor chip was optimized for the illumination system from rear panel and the detection sensitivity of the system under the back illumination was compared with that for the other conditions including the top illumination system.