Impact of Hyperglycemic Control on Left Ventricular Myocardium A Molecular and Cellular Basic Study in a Diabetic Rat Model

Pei-Hsun Sung; Cheuk-Kwan Sun; Sheung-Fat Ko; Li-Teh Chang; Jiunn-Jye Sheu; Fan-Yen Lee; Chiung-Jen Wu; Sarah Chua; Hon-Kan Yip

doi:10.1536/ihj.50.191

Impact of Hyperglycemic Control on Left Ventricular Myocardium

A Molecular and Cellular Basic Study in a Diabetic Rat Model

Pei-Hsun Sung ^†, Cheuk-Kwan Sun ^†, Sheung-Fat Ko ^†, Li-Teh Chang ^†, Jiunn-Jye Sheu ^†, Fan-Yen Lee ^†, Chiung-Jen Wu ^†, Sarah Chua ^†, Hon-Kan Yip ^†

Author information

Pei-Hsun Sung ^†
Department of Internal Medicine, Chang Gung Memorial Hospital-Kaohsiung Medical Center, Chang Gung University College of Medicine
Cheuk-Kwan Sun ^†
Department of General Surgery, Chang Gung Memorial Hospital-Kaohsiung Medical Center, Chang Gung University College of Medicine
Sheung-Fat Ko ^†
Department of Radiology, Chang Gung Memorial Hospital-Kaohsiung Medical Center, Chang Gung University College of Medicine
Li-Teh Chang ^†
Basic Science, Nursing Department, Meiho Institute of Technology, Pingtung nnn, Department of Veterinary Medicine, National Pingtung University of Science and Technology
Jiunn-Jye Sheu ^†
Department of Cardiovascular Surgery, Chang Gung Memorial Hospital-Kaohsiung Medical Center, Chang Gung University College of Medicine
Fan-Yen Lee ^†
Department of Cardiovascular Surgery, Chang Gung Memorial Hospital-Kaohsiung Medical Center, Chang Gung University College of Medicine
Chiung-Jen Wu ^†
Division of Cardiology, Chang Gung Memorial Hospital-Kaohsiung Medical Center, Chang Gung University College of Medicine
Sarah Chua ^†
Division of Cardiology, Chang Gung Memorial Hospital-Kaohsiung Medical Center, Chang Gung University College of Medicine
Hon-Kan Yip ^†
Division of Cardiology, Chang Gung Memorial Hospital-Kaohsiung Medical Center, Chang Gung University College of Medicine
Author contributions

†These authors contributed equally: Pei-Hsun Sung, Cheuk-Kwan Sun, Sheung-Fat Ko, Li-Teh Chang, Jiunn-Jye Sheu, Fan-Yen Lee, Chiung-Jen Wu, Sarah Chua, Hon-Kan Yip

Keywords: Hyperglycemia, Inflammation, Left ventricular dysfunction

JOURNAL FREE ACCESS

2009 Volume 50 Issue 2 Pages 191-206

DOI https://doi.org/10.1536/ihj.50.191

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Abstract

This experimental study investigated the impact of hyperglycemic control on left ventricular (LV) function using a model of diabetes mellitus (DM) (induced by streptozocin 60 mg/kg). Sixteen adult-Sprague Dawley rats were divided into group 1 (poor hyperglycemic control, n = 8) and group 2 (good hyperglycemic control, n = 8). Diabetic rats and 8 healthy rats serving as controls (group 3) were sacrificed on day 28 after DM induction. The results demonstrated that HbA_1C on day 28 was higher in group 1 than in groups 2 and 3 (P < 0.0001). The mRNA expressions of MMP-9 and endothelin-1 were elevated in group 1 compared with that in groups 2 and 3 (P < 0.05), whereas PGC-1α and eNOS were lower in group 1 than in groups 2 and 3 (P < 0.05). The number of apoptotic nuclei was higher in group 1 than in groups 2 and 3 (P < 0.01). The integrated area (μm²) of connexin43 (Cx43), Cx43 protein expression, and LV function were lower in group 1 than in groups 2 and 3 (P < 0.05). Moreover, PKC-ε expression in the mitochondrial compartment was decreased in group 1 compared to that in groups 2 and 3 (P < 0.005).

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