Intracellular Sodium Concentration in Diabetic Rat Ventricular Myocytes

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We measured intracellular Na⁺ concentration ([Na⁺]i) of diabetic rat ventricular myocytes using sodium-binding benzofuran isophthalate (SBFI). We used diabetic rats at 8 weeks after the injection of streptozotocin (45mg/kg i.v.). The level of [Na⁺]i during the control perfusion was significantly lower in diabetic myocytes than that in normal myocytes (9.2±0.4mM v.s. 12.0±0.3mM, p<0.01). After the 40min perfusion of 1μM hexamethylene amiloride (HMA), [Na⁺]i decreased significantly in both groups. However, there was no difference in [Na⁺]i between the two groups after the perfusion of HMA. It was suggested that the lower [Na⁺]i in diabetic myocytes could be due to the decreased activity of Na⁺/H⁺ exchange system. (Jpn Heart J 36: 647-656, 1995)