Efficiency of Crude Extract from Pummelo Peel on Controlling the Growth of Colletotrichum gloeosporioides (Penz.)

Abstract

The objective of this research was to investigate the antifungal efficiency of pummelo peel extracted with different solvents on Colletotrichum gloeosporioides. Crude extracts from pummelo peels were eluted with four solvents, such as ethanol, hexane, dichloromethane and ethyl acetate. Chemical compounds of the crude extracts were analyzed by GC-MS and HPLC and inhibition the growth of C. gloeosporioides was tested by PDA culture. The result showed that limonene was the major compound in all extracts (79.55-89.91%). α-Phellandrene and 2, 6-octadien-1-ol, 3, 7-dimethyl were found only in crude extracts using ethyl acetate as a solvent. In addition, the extract was eluted with ethyl acetate also had the amount of flavonoids: nobiletin and 3-hydroxy-7, 3’, 4’, 5’- tetramethoxyflavone, higher than those eluted with other solvents. Inhibition the growth of C. gloeosporioides of pummelo peel extracted with dichloromethane, ethanol, hexane and ethyl acetate were 19.16, 52.81, 65.58, and 95.61%, respectively. Therefore, pummelo peel extracted with ethyl acetate was the highest efficiency on controlling C. gloeosporioides (p ≤ 0.05).