抄録
The purpose of this study was to compare the production of interleukin-1β (IL-1β) by peptidoglycan from Lactobacillus casei (L. casei) in human pulp-derived fibroblasts from deciduous (DHPF) and permanent teeth (HPF). DHPF and HPF were collected from 6 noncarious deciduous teeth (6-9 year-old individuals) and 6 noncarious permanent teeth (20-25 year old individuals) extracted in the course of orthodontic treatment. Both IL-1β protein production and IL-1β mRNA levels from peptidoglycan- treated DHPF and HPF were clearly enhanced, compared to IL-1β protein production and IL-1β mRNA levels from peptidoglycan-non-treated DHPF and HPF that were not treated by peptidoglycan. Furthermore, IL-1β protein production and IL-1β mRNA levels from peptidoglycan-treated DHPF showed clearly lower levels, compared with IL-1β protein production and IL-1β mRNA levels from peptidoglycan-treated HPF. These findings suggest that pulpitis in deciduous teeth was less induced than pulpitis in permanent teeth, and that L. casei- peptidoglycan stimulated IL-1β production through enhancement of IL-1β mRNA expression from DHPF and HPF.
