Cytotoxic effect of Aggregatibacter actinomycetemcomitans Lipopolysaccharide on Human Leukemia Cell Lines and Human Gingival Fibroblasts

抄録

Aggregatibacter actinomycetemcomitans is implicated in the etiology of aggressive periodontitis and chronic periodontitis. Lipopolysaccharide (LPS) is a major constituent of the outer membrane of Gram-negative bacteria. LPS from Aggregatibacter actinomycetemcomitans (Aa-LPS) and Escherichia coli (Ec-LPS) was isolated by the hot phenol-water method and partially purified on a column. The purpose of this study was to compare the properties of Aa-LPS with Ec-LPS. Aa- and Ec-LPS showed different patterns in the results of SDS-PAGE. Furthermore, the total sugar and limulus activity of Aa-LPS were different from those of Ec-LPS. The cytotoxic effect of Aa-LPS was in a time- and dose-dependent manner. Aa-LPS had a strong cytotoxic effect on human leukemia cell lines and human gingival fibroblasts compared with Ec-LPS. These results suggested that the difference in structure, total sugar amount and limulus activity of Aa-LPS from those of Ec-LPS or the cell difference may participate in the cytotoxic effect.