2017 年 16 巻 1 号 p. 1-7
Plasma rich in growth factors(PRGF) can be rapidly obtained from patient blood. PRGF has been used in regenerative therapy for soft tissue and bone formation, and represents a new and potentially useful adjunct in oral and maxillofacial bone reconstructive surgery. However, few studies have investigated the biological functions of PRGF in bone regeneration. Human mesenchymal stem cells(hMSC) isolated from human bone marrow have the capacity to commit to multiple cell types such as the osteoblastic lineage, and have been widely applied in tissue engineering studies in recent years. The aim of this study is to evaluate the effects of PRGF for osteogenic differentiation in hMSC. PRGF was prepared from whole blood centrifuged at 460×g for 8 min. PRGF F2 was incubated with 10% calcium chloride solution at 37℃ for 1 h to trigger platelet activation and growth factor release. Activated PRGF Fraction 2 was centrifuged at 3,000×g for 15 min, and the supernatant was then isolated. We examined the effects of soluble factors in PRGF on proliferation and mineralization in hMSC culture supplemented with PRGF. The proliferation of hMSC was increased in osteogenic induction medium(OIM) supplemented with PRGF. Alkaline phosphatase activity increased in hMSC by PRGF. Staining for alizarin red S and von Kossa was strong in hMSC supplemented with PRGF. These results suggest that PRGF is able to promote bone generation.
