Manganese Stimulates Ca²⁺ Mobilization in Human Gingival Fibroblasts

抄録

In fura-2-loaded human gingival fibroblasts, 1-50 μM MnCl₂ evoked an increase in intracellular Ca²⁺ concentrations ([Ca²⁺]_i) in a dose-dependent manner. This increase was abolished in the absence of extracellular Ca²⁺. MnCl₂ induced an increase in [Ca²⁺]_i provoked prostaglandin E₂ release in human gingival fibroblasts in time- and dose-dependent manners. MnCl₂ failed to evoke prostaglandin E₂ release in the absence of extracellular Ca²⁺. A protein kinase C activator and a tyrosine kinase inhibitor inhibited the increase in [Ca²⁺]_i induced by MnCl₂. These results suggest that Mn²⁺ evokes Ca²⁺ entry and prostaglandin E₂ release in human gingival fibroblasts, which are regulated by protein kinase C and tyrosine kinase activities.