DOSE-RESPONSE RELATIONSHIP IN DELAYED TYPE CONTACT SENSITIVITY WITH MANEB: AN EXPERIMENTAL MODEL OF "SIMPLE CHEMICALS"

Abstract

As an experimental model of dose-response relationship in contact sensitivity with simple chemicals, maneb, which has been known to be a strong sensitizer, was chosen and its modalities of sensitization in the guinea pig was investigated.
As a result, dose-dependent relationship between induction and sensitization response was apparently indicated by intradermal injection as well as topical ap-plication technique. The skin reactivity was also corresponded with a range of concentrations used for challenge, but too low challenge concentrations represented a failure to respond despite the fact that the animals were indeed sensitized. A regression line was calculated and about a linear relationship between the induction dose and the incidence of sensitization was obtained from the semilogarithmic graph.
The importance of topical induction area and repetitive application in contact sensitivity was also examined and discussed.