Abstract
An automated method for the determination of adrenaline and noradrenaline in the eluate from alumina is presented. In this method, citrate was added to the alumina eluate to prevent the interference of aluminum hydroxide produced at neutral pH for the determination. For the differential estimation of the two catecholamines, two filter sets for activation and emission in fluorometry were used. The optimum concentrations of reagents for the determination were investigated.
Almost all catecholamines in the acetate buffer solution were recovered by the present method, and the recovery of catecholamines added to the alumina eluate of human urine was 90 to 95%. Correlationship between the data obtained by the method of Euler and Lishajko1) and those by the present method was as follows; coefficients of correlation were 0.941 and 0.978 and regression lines were y=0.86x+4.4 and y' = 1.05x'-2.4 for adrenaline and noradrenaline respectively (N=50, y y' for the automa-ted method and x, x' for the manual method).
