1976 年 15 巻 2 号 p. 111-114
The kidney and intestine are considered to hold definite positions in the bilirubin metabolism, especially during obstructive jaundice. In this study, renal and intestinal cytoplasmic binding proteins of organic anions, as well as hepatic ones, were analyzed by Sephadex G 75 column chromatography, using 131-I sulfobromophthalein. The enzyme activities of glutathione S-transferases in those organs were also measured using di-nitrochloro- benzene as a substrate, since some of the cytoplasmic binding proteins are glutathions S-transferases synonyms. Hepatic, renal and intestinal ligandins were compared, by these methods, with each other between two groups: control and bile duct ligated rats. In the bile duct ligated rats, the binding increased in the kidney, and remained unchanged in the intestine and liver. But enzyme activity had a tendency to decrease in all the three organs.
