抄録
Anin vitro method for the determination of erythropoietin (EPO) titres in humanserum samples was developed to detect low levels of EPOtitres in various haematological states. Fetal mouse liver cells (FMLG)were incubated in methylcellulose-containing media, and radioiron incorporation into haem was estimated in cultured FMLC.Wecould determine the concentrations of EPOfrom around 20 to 600 mU per ml of a very small amount of serum samples from patients and normal adults. Using this method, EPO titres of normal adults (54±31 mU/ml), chronic renal failure patients (29±23 mU/ml), aplastic anaemia patients (545±74 mU/ml), and polycythaemia vera patients (16±7 mU/ml) were determined.
