Abnormalities in Platelets and Vascular Endothelial Cells Induced by Glycated Lipoproteins

Abstract

We studied the effects of glycated lipoproteins of low-and high-density (LDL and HDL) on platelets and vascular endothelial cells. After pretreatment for 5 minutes at 37°C, the thrombininduced synthesis of thromboxane B₂ in washed platelets was significantly increased by glycated LDL as compared with native LDL (198.9 ± 16.2 vs 90.3 ± 29.4ng/10⁹ platelets, n = 8, p < 0.01). Platelet aggregation was also increased by glycated LDL as compared with native LDL. After treatment with platelet-rich plasma for 5 hours at 37°C, these values were suppressed by native HDL vs the control (buffer), but not by glycated HDL. Abnormalities in the release of 6-keto prostaglandin F₁α and lactate dehydrogenase from vascular endothelial cells were also induced by glycated LDL and/or HDL. These observations suggest that abnormalities induced in platelets and vascular endothelial cells by glycated lipoproteins may play an important role in the development of atherosclerosis in patients with diabetes mellitus.
(Internal Medicine 31 : 746-751, 1992)