The Two-Color Analysis of the T-Lymphocyte Subsets in Experimental Allergic Neuritis in Rats

Abstract

We examined the T-cell subsets in the peripheral blood, spleen and lymph nodes during the course of experimental allergic neuritis (EAN) by using two-color analysis. In the acute phase, the percentages of CD4⁺ major histocompatibility complex (MHC)-II⁺ cells and CD8⁺ MHC-II⁺ cells in the lymph nodes of EAN rats were significantly higher than in the control rats. In the recovery phase, the percentages of CD4⁺ CD45RC⁺ cells and CD8⁺ CD45RC⁺ cells in the lymph nodes in EAN rats were significantly higher than in the control rats. However, significant changes of the T-cell subsets were not detected in either the spleen or the peripheral blood during the course of EAN. These results suggest that CD4⁺ MHC-II⁺ cells, CD8⁺ MHC-II⁺ cells, CD4⁺ CD45RC⁺ cells and CD8⁺ CD45RC⁺ cells may play a role in the course of EAN. The relationship between these double staining cells and EAN is discussed.
(Internal Medicine 34: 347-351, 1995)