Abstract
Erythrocytes can adsorb various substances and thereby be rendered specifically agglutinable by serum antibody directed against the substance adsorbed. The author carried out the agglutination of goat erythrocytes which adsorbed myocardium phosphatide.
1) Direct method; one volume of 0.5% suspension of erythrocytes, which adsorbed myocardium phosphatide (0.5mg/ml) was added to one volume of serum dilution, from which normal hemagglutinin was removed. This mixture was incubated for 2 hours at 37°C and reading was made by 12 hours at room temperature.
2) Tannic acid method; one drop of 2.5% suspension of sensitized erythrocytes treated with 1:5, 000 tannic acid in normal rabbit serum dilution (1:100), from which normal hemagglutinin was removed, was titrated to the patients' serum dilutions. This mixture incubated as above, and rearding was conducted by 12 hours in a refrigerator.
Amounts of the myocardium phosphatide, which was necessary to sensitized erythrocytes, was enough by the use of 0.1mg/ml dilution. Tannic acid was also enough by the use of 1:20, 000 dilution. The author assertained the existence of myocardium phosphatide antigen in human sera by these hemagglutination techniques. Patients with myocardial damages showed higher titer than those without myocardial damages. A relation was found between the hemagglutination and the myocardium phosphatide precipitation. If myocardium phosphatide was added to the decholan solution, the hemolysis became intensive, and thereby the hemagglutination became almost impossible, but the final titer was the same.
