Abstract
The author stained trichomonas elongata with fluorescent pigments, auramin and Acridinorange, under various conditions, and observed morphologically and biologically through fluorescent microscope. The present paper deals with a part of the studies with auramin.
In case of smear, auramin diluted by 4, 000 to 6, 000 times with 2 to 4% of phenol was suitable for the observation of detailed structures of trichomonas elongata. However, fluorescence of their flagella was very faint. In order to observe the lengths and numbers of glagella, concentration of phenol should be 3% or 4% and that of auramin should be 1: 2, 000 to 1: 4, 000
In case of float preparation, auramin was required to be diluted 4, 000 times to 10, 000 times with 4% to 5% of phenol. It was also found that 5% to 10% of formalin should be added to the observed materials. Extremely minute fluorescence granules were found in the bodies of trichomonas elongata. Furthermore, regularly arranged chamber-like structure, seen similarly as bamboo joints, were found in the flagella. These “joints” were seen strongly fluorescence. The essentials of the “joints” were not clasified.
