On the occasion of the guide-revision of the the-bac, examination-method, the research association was concluded for the purpose of re-examination of the customary technics and of planning the technic standerizing of the method.
The results were as follows:-
1) The Kokuryo-type shaker was invented for mixing of sputum.
2) The results of inoculation of 1% HaOH sputum are apparently improved by using this shaker.
3) For promoting efficiency of culture manipulation, 6-7 days long horizontal situation of culture medium following gumcapping direct after inoculation was tried and was proved as usefull as customary methods.
4) Concerning the culture of the-bac, in gastric juice, the treatment with 4% NaOH is more usefull than the others.
5) For diminution of resistance culture titre through long time incubation there is no fear as far as the culture is selfmade.
6) As to SM and PAS, resistance is higher estimated on the 3% Ogawa culture than on the 1% culture. But to INH no differences.
7) Resistance titres of the same samples, estimated by each laboratory with indirect method, differed much more than expected. So the causes of this incoincidence will be investigated by this association.