Abstract
It was found in ordinary clinical examination utilizing 1314TH resistant media (1% Ogawa's media) that the minimal inhibitory concentration of tubercle bacilli often varied from expectation. Accordingly, experiments were conducted on the influence of sterilization temperature during media production and of time lapse after making of the media, and on minimal inhibitory concentration. Results show that the titer drops after one month; and that media produced at a temperature of 80 degrees for one hour, shows a smaller degree of lowered titer than media produced at a temperature of 90 degrees for one hour.