抄録
For several years the bone marrow functions have been studied by using rabbits by Ito's new methods.
Materials and methods
Female rabbits, weighing 2, 500 to 3, 000g, were used.
1. The rabbits were affected by infections, phlebotomy, hemolysis, splenectomy, steroid hormon administration and so on.
2. After placing a polyvinyl catheter into the nutrient vein of the femur, the
rabbits were affected by phlebotomy, hemolysis, steroid hormon administration or I.V. adrenalin and then the release of blood cells were studied.
3. Blood samples were obtained from the nutrient vein of the femur of the rabbits treated with 59Fe and ferrokinetics were studied.
4. Blood samples obtained from the nutrient vein of the femur of the rabbits with infection were incubated in vitro for 1 to 5 hours with 59Fe or 3H-thymidine, and examined with microradioautographic technics.
5. After injection of indian ink into the femur bone marrow of one side or Coirradiation 3H-thymdine was administered intravenously to the rabbits, and blood samples obtained from the bilateral femur bone marrows were examined with microradioautographic technics.
The patients with infection (pulmonary abscess, pueumonia) and blood disease (leu-kemia, iron deficiency anemia, polycythemia, pernicious anemia, idiopathic thrombopenic purpura) comprised the substance of this study.
Bone marrow aspirates obtained from the patients were incubated in vitro for a short time with 59Fe or 3H-thymidine, and the grain counts of labeled cells were examined.
The results of this experimental study differed from those of the clinical studies. These results suggest that the blood cells issuing from the bone marrow under morbid conditions alter while they circulate through the whole body.
From this study some possibilities of the alterations of the hemopoiesis and the release of blood cells under morbid conditions were discussed.
