Abstract
Using pre-incubation with [35S] methionine followed by extraction with petroleum ether, it was shown that cultivated tumor cells including HA-60 (acute promyelocytic leu-kemia cell-line), K-562 (chronic myeologenous leukemia cell-line) and NKM-1 (acute my-elomonocytic leukemia cell-line), contained sulfolipid. Such sulfolipid was not found in normal lymphocytes and mouse organs. The liver of mice did not contain sulfolipid read-ily extractable with petroleum ether after extraction with a trichloroacetic acid solution, but it contained sulfolipid that was liberated by hydrolysis with potassium hydroxide-methanol solution.
