Abstract
We typed HLA-DRB1, B3, B4, B5, DQA1, and DQB1 allele specificities by the PCR-SSO method in 200 hemodialysed patients who had done or were in a waiting list for kidney transplantaion in Sakura National Hospital. An antigen frequency of DR2 (DRB1*1501) was significantly low (p<0.05) and that of DR4 (DRB1*0405) was high (p<0.01) in HD group when they were compared with those of 235 healthy controls. A strong linkage disequilibrium between DR and DQ alleles was observed but none of special DR-DQ combinations in the HD group was noticed. A total of 44 estimated haplotypes was found in two groups.
We calculated HLA-DRB1 (HLA-D) matching ratios between HD patients and controls for DR specificities that are used for recipient selection. The ratios were 100% for DR1, DR7, DR9, and DR10, 90% for DR13, approximately 50% for DR2, DR8, and DR12, 41% for DR4, and 22%, the worst, for DR14.
