Abstract
The preliminary results of the currently established cord blood bank in Kure National Hospital have been reported. Seventy-eight cord blood (CB) samples were collected during delivery of the placenta. Among 78, we used the first 25 umbilical cord bloods for the technique acquisition. Twenty-five fresh CB samples had a mean volume of 62ml. After volume reduction, the respective CB units contained 508×106 uncleated cells (NC), 204×106 mononucleated cells (MNC), 64×104 CFU-GM, 18×104 BFU-E and 2×106 CD 34+cells. Therefore, the overall recovery for NC and MNC was 67% and 59%, respectively. On the other hand, 53 fresh CB samples for the clinical use had mean volume of 72ml, After volume reduction, the respective CB units contained 615×106 NC, 309.0×106 MNC, 78×104 CFU-GM and the overall recovery for MNC was 79%. Results of these 53 cord bloods are better than those of the first 25 units used for technique acquisition. Bacterial contamination of collections has been reduced from 23% of 25 units to 3.8% of 53 units. The results of NC, MNC, CD34 positive cell, CFU-GM and BFU-E counts are good enough for clinial use
