細菌酵素に及ぼす抗菌物質の作用
(第3報) Homosulfamin耐性菌株の蛋白分解酵素作用について
1952 年 6 巻 6 号 p. 377-381
詳細
1952 年 6 巻 6 号 p. 377-381
1) Studies were carried out on the protease actions of acetone powder of 3 strains of Stap-hylococcus and 2 of Escherichia coli that had been made marfanil resistant.
2) The optimum PH for hydrolysis of peptone and casein was approximately 8.0.
3) Ths proteolytic action of these resistant strains was found to be remarkably weaker than that of the original strains.
4) While the protease actions of the original strains of these bacteria were notably inhibited by the addition of marfanil, the resistant strains were scarcely influenced by it except a single strain of Staphylococcus tested. However, they were considerably inhibited by furacin (5-nitro-2-furfuralsemicarbazone).
5) It was not proved that the suspension of the resistant strains had enzyme actions to liberate ammonia from marfanil.
6) The anti-marfanil factors of the rtesistant strains were readily inactivated by the thermic treatment (50°C. 30 minutes over)These findings might suggest that anti-marfanil tendency of the protease actions of the resistant strains would be responsible for one of the important factors in the aquisition of bacteria resistance.
