抄録
To make research cytogenetic properties of commonly distributed species in East Eurasian Flora related to Flora of Japan, the chromosome numbers and karyotypes in two species and one subspecies of Achillea in Japan and Russia were investigated by orcein and fluorescent sequential staining of GC-specific chromomycin A3 (CMA) and AT-specific 4', 6-diamidino-2-phenylindole (DAPI) banding methods. Achillea alpina subsp. subcartilaginea, A. asiatica and A. millefolium showed the tetraploidal and hexaploidal chromosome numbers of 2n=36, 56 and 36, respectively. Unexpected odd satellite chromosome (sat-chromosome) numbers were shown in the tetraploid species of A. alpina subsp. subcartilaginea and A. millefolium. In contrast, six sat-chromosomes were observed in the hexaploid A. asiatica. Additionally, median-centromeric chromosome did not have any satellite. All sat-chromosomes of the both tetraploid species were submedian-centromeric chromosomes, while all sat-chromosomes of the hexaploid species were subterminal-centromeric chromosomes. The satellites were well characterized as CMA-positive and DAPI negative sites.
