Micropropagation and chromosome stabilities of four endangered species of orchids in Indonesia

抄録

The combination of BA and NAA in concentration at 0-4 mg/l could not significantly induce shoot primordia in four endangered species of orchids. Size of shoot meristem used in the preliminary research might be too big to induce shoot primordia in Grammatophyllum speciosum and for increasing the survival frequency of Vanda and Phalaenopsis. Certain concentrations of BA and NAA affected the survival frequency of Vanda and Phalaenopsis. The type of basal medium has no effect to the growth and the survival frequency of the orchid studied, except for Phalaenopsis gigantea which only grow well in Hyponexpepton medium. The chromosome numbers of the four orchid species showed very high stabilities during the courses of their micropropagations.