抄録
Cupressus sempervirens L. had the chromosome number of 2n=22 and its karyotype was composed of 22 metaand submeta-centric chromosomes with gradual variation in their lengths. In the chromosome complement six chromosomes had a secondary constriction at proximally interstitial region of the short arm and had CMA-bands on same positions of all secondary constrictions and neighbor regions. After silver staining six Ag-NORs appeared on nucleoli and chromosome at telophase, and at metaphase Ag-NORs appeared at the secondary constrictions of a few chromosomes. These results indicate all CMA-banded secondary constrictions have nucleolar organizing function. DAPI-bands appeared at interstitial and proximal regions of some chromosomes. A combination of chromosome morphology and fluorescent band patterns of the CMA- and DAPI-stainings can identify all pairs of homologous chromosomes. Advantage of the fluorescent bandings for comparative karyotype analysis in Cupressus is discussed.
