Advanced Digital Pathology : Whole Tissue Imaging and Whole Block Imaging

Abstract

Traditional histologic methods are hindered by glass slide creation through tissue sectioning, staining, and additional scanning. Traditional whole slide Imaging scanner also only provides two-dimensional (2D) image representations of three-dimensional structures. Micro-computed tomography (micro-CT) opens a disruptive imaging opportunity, not only in obviating the need for sectioning and tissue staining but also in rendering three-dimensional (3D) images useful for new morphologic insights. Whole Block Imaging/Whole Tissue Imaging using micro-CT allows for deeper analysis than originally anticipated. Now, the tumor/tissue volume and location through Whole Block Imaging (WBI)/Whole Tissue Imaging (WTI) can be identified, then request diagnostic areas for slide preparation. This development reduces cost and the potential risk of losing tissue that may arise through traditional method. Therefore, we have evaluated micro-CT technology and feasible clinical utilities in pathology. AI and computational technology must play the important roles to accelerate interpretation and to improve the quality in further development.