SUPPRESSIVE EFFECT OF DIODE LASER IRRADIATION ON PICRYL CONTACT SENSITIVITY

抄録

Although recent studies show that low reactive-level laser irradiation has some effects on several immune responses, few studies have examined the effect of diode laser irradiation on picryl contact sensitivity, a kind of cellular immunity. This study consisted of three experiments to evaluate the effect of diode laser irradiation (DLI) on picryl contact sensitivity in mice. First, (Exp.1), the laser irradiation site (right ear) was the same as the challenge site (right ear). Second, (Exp.2), the irradiation site (proximal portion of tail) was remote from the challenge site (right ear) Third, (Exp.3) we evaluated the penetration of diode laser energy through the irradiation site (right ear) compared with a range of incident powers. The effect of DLI for picryl contact sensitivity was evaluated with measurements of any increase of the ear thickness. The irradiation times in Exp.1 and Exp.2 were 15, 30 and 60 min. The results showed that DLI suppressed the cutaneous inflammation of picryl contact sensitivity in an exposure time dependent manner and this suppressive effect was restricted to within the irradiation site. 60 min irradiation in the challenge site was remarkably effective, however irradiation of the remote site did not affect the ear thickness increments in the challenge site. The penetration of laser energy through the ear was almost 50% of its output power in the whole range of powers tested.