CHANGES IN FIBROBLAST PROLIFERATION AND METABOLISM FOLLOWING IN VITRO HELIUM-NEON LASER IRRADIATION

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Fibroblast viability, proliferation and metabolic changes were studied following in vitro irradiation with a continuous wave 10 mW helium-neon (HeNe) laser, Fresh first generation fibroblasts prepared directly from a healthy human skin sample were used in the culture. and a control set of unirradiated samples was handled in exactly the same way as the irradiated samples. Statistical analysis of the data showed no increase in the number of fibroblasts following low reactive-level laser therapy (LLLT) levels of radiation, but did reveal significant changes in metabolic rates compared with the unirradiated controls, Results of the assessment of the metabolic changes suggested that an efficient modality for enhancement or modulation of cell activity could be ascertained for laser irradiation at LLLT levels, and thereafter used for therapeutic purposes, following the elucidation of appropriate power and energy densities, and other parameters.