CONSTRUCTION OF SUBTRACTED OSTEOBLAST cDNA LIBRARY WITH LASER-IRRADIATION-ENHANCED TRANSCRIPTION

抄録

We examined an approach in the cloning of the vast majority of those osteoblast mRNA whose transcription was highly induced when the osteoblasts were irradiated with a GaAlAs diode laser. To identify the genes induced by laser irradiation, a stepwise subtraction procedure was carried out between laser-irradiated and non-irradiated MC3T3-E1 cells. Several clones exhibited high homology with mitochondrial protein- and DNA replication related-genes. Our strategy of subtraction cloning is a powerfu1 tool for isolating the specific laser-irradiated MC3T3-E1 cells or highly induced cDNAs. This approach is useful for studying the mechanisms of cell response following bioactivation with low incident levels of laser irradiation.