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This paper describes a new method for tracking objects in 3D motion from image sequences by constraints in the scene. Many methods based on the gradient scheme have been proposed for tracking of objects. However, these methods have a serious drawback, in which tracking error sometimes increases during a long image sequence due to the various noise. The proposed method enables us to track the objects in long time by the gradient-based constraint with some scene constraints, that is, constraints on position, velocity, and acceleration from scene. Experiments in real image sequences support the precise tracking in long time.