Studies on the pathogenicity of Candida albicans mannan in denture stomatitis.

Abstract

Candida albicans (serotype A) T strain was isolated from a patient with denture stomatitis, and polysaccharides (crude polysaccharide, crude mannan, neutral mannan and acidic mannan) were extracted from the cell walls of the C. albicans T strain. A delayed skin reaction test and macrophage migration inhibition test were performed using the guniea pigs sensitized with the whole cells of C. albicans T strain.

Several fractions from C. albicans T strain were used as an antigen. A permeability increasing factor test was also performed in order to study inflammatory activity. In addition, experimental denture stomatitis was induced by inoculating crude polysaccharide under an acrylic plate. The results were as follows;

1. Crude polysaccharide was exhibited delayed skin reactivity. All extracted fractions were exhibited macrophage migration inhibitory activity.

2. All the extracted fractions were demonstrated to possess the permeability lncreasing activity.

3. Histologically, the chronic inflammation phase was observed in the palatal mucosa.

These results suggest that C. albicans mannan is the cause of denture stomatitis.