A study on the angioarchitecture after auto-rib grafting to irradiated mandibular bed of the rats.

Abstract

The effect on the angioarchitecture of bone grafts in the mandibular region after irradiation was investigated. Bridging rib grafts were carried out in 80 rats on the 14th day after irradiation with electron beam at 5-,10-,20-and 30Gy, and on 8 rats without irradiation. Transparent preparations of India ink and H. E. stain, as well as measurement of the vascular level showed the following results:

After irradiation of 5-and 10Gy or 20-and 30Gy, the body weight showed a phasic or a biphasic pattern. Howship lacunae appeared in the compact bone on the 7th and 14th day. New vessels penetrated into the bone marrow via Volkman’s and Harversian canals, and new bones were formed there. On the 30th day, new bone apositions on the inner or outer compact bone, a constriction of the bone marrow and a bridge between the recipient and the graft were observed. On the 60th day, new vessels and bones were actively formed at the periostium. The bridging bone became thick and the sinusoid-like vessels of the bone marrow expanded. Vascular levels of the compact bones and the bone marrow in both the non-irradiation and the irradiation groups gradually showed a tendency to increase on the 7th, 14th and 30th day, but decreased on the 60th day. However, only the bone marrow level increased even on the 60th day. On the other hand, at 5-and 10Gy most of the bone marrow levels showed a tendency to be lower than in the non-irradiation group, but at 20Gy most levels were near the levels of the control group.