核酸クロマト型チップを用いた大麻DNA検査キットの開発と実証評価

抄録

　The forensic identification of cannabis is performed by a combination of chemical analysis and morphological examination. Recently, molecular biological analysis using cannabis DNA information has been noticed as a new approach. In this study, the cannabis DNA detection kit using a DNA chromatography chip was developed, and the demonstration evaluation in the forensic chemical laboratory was carried out.

　The DNA detection kit of a “four-line version” which had the function to distinguish fiber-type from drug-type cannabis showed as high accuracy (98.3%) as the current identification method on cannabis identification. However, there was a tendency to mistake a part of the drug-type samples as “fiber-type cannabis”. In the kit of a “three-line version” which was specialized for the cannabis DNA detection, the accuracy of 99.0% was confirmed on the cannabis identification. There were no false positives throughout all evaluations. In addition, some of the combustion residues that could not be identified as cannabis by the current identification method were classified to be “cannabis positive” by the DNA detection kit, indicating the effectiveness of a new approach.

　As a result of this study, it was shown that the quick and accurate cannabis DNA analysis could be carried out by the DNA detection kit even by analytical chemists who didn't have expertise in molecular biology.