日本法科学技術学会誌
Online ISSN : 1881-4689
Print ISSN : 1880-1323
ISSN-L : 1880-1323
QuEChERS法及び水素炎イオン化検出ガスクロマトグラフィーを用いる食品中Δ9-THCの定量試験法の検討
城 克己家宇治 啓倉上 大祐津村 ゆかり
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論文ID: 817

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 We have developed a quantitative method that uses GC-FID and QuEChERS to measure Δ-9-tetrahydrocannabinol (Δ9-THC) in cannabis-infused foods. Reported methods for the analysis of cannabis-infused foods typically use liquid chromatography, but obtaining Δ9-THC used in these methods, with the certified concentration, is difficult in Japan. Therefore, we attempted to apply a method, obtained from the United Nations Office on Drugs and Crime, that allows the quantitative analysis of Δ9-THC in herbal cannabis, hashish, and liquid using cannabinol as the reference material. In this study, the QuEChERS method, which is widely used in food analysis, was adopted. The EN 15662 salt method (EN salt method) and Enhanced Matrix Removal-Lipid method (EMR-Lipid method) were compared using recovery tests for Δ9-THC added to four types of food samples. The EN salt method was found to be superior to the EMR-Lipid method for some foods. A similar test was conducted on six different types of foods using the EN salt method. The recovery was 95.2% to 113.5%. The precision was tested at two levels of Δ9-THC content in three trials over three days. The intra-day precision was 9.8% and 1.4% for 0.1 mg/g and 1 mg/g Δ9-THC, respectively. The inter-day precision was 16.6% and 4.4% for 0.1 mg/g and 1 mg/g Δ9-THC, respectively. Cannabis-infused brownie was homogenized, divided, and tested in three laboratories. The average values of Δ9-THC content at the three laboratories were 0.33, 0.43, and 0.48 mg/g. We analyzed Δ9-THC content in 13 cannabis edibles seized in Japan. The overall Δ9-THC content ranged from 0.33 mg/g to 7.14 mg/g, and the content per piece ranged from 4.3 mg to 13.9 mg. This study demonstrates a useful method for the quantification of Δ9-THC in cannabis-infused foods.

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