Fast Blue BBによる呈色および薄層クロマトグラフィー（TLC）を用いた 大麻現場試験法の開発（第二報）

抄録

In this study, we investigated the stability of reagents and improvements to the kit. Regarding improvements to the kit, we were able to develop a kit that is more suitable for on-site use by using a plate with a concentration zone and a spreading chamber.

To examine stability, Fast Blue BB (FBBB) was dissolved in chloroform and stored at room temperature under light-shielded and non-light-shielded conditions, and TLC was performed after 2, 7, 14, 21, and 28 days. As a result, although it could be used for up to 2 days after storage without light shielding, normal color development was not observed after 7 days, and even when TLC was performed, no Δ⁹-THC spots could be confirmed. On the other hand, for those stored in the dark, a normal color reaction was observed even 28 days after preparation, and a spot could be observed in the same position as the standard solution on TLC.

After the standard solutions were prepared, they were stored for 7, 14, and 28 days under four storage conditions (room temperature, shielded from light, drying, and shielded from light + drying). When TLC was performed for each storage condition, it was confirmed that all the products could be used for the specified period of time without any problems.