THE DISSOLUTION EFFECT OF SODIUM HYPOCHLORITE AND DENATURATION OF THE STRUCTURE OF (PRO-PRO-GLY) 10 USING ELECTRON SPIN RESONANCE AND NUCLEAR MAGNETIC RESONANCE
2012 Volume 3 Issue 2 Pages 75-83
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2012 Volume 3 Issue 2 Pages 75-83
Sodium hypochlorite (NaClO) is used for root canal cleaning to dissolve organic tissue. However, the detailed mechanisms of dissolution of organic tissue have not yet been clarified. The aim of this study was to examine the mechanisms. Gelatin gel was interacted with 0-5% NaClO for 1-60 minutes. Then, we investigated weight of the remaining gelatin gel, pH, free radicals using electron spin resonance, and the relationship between free radicals and the amount of dissolution.
A high concentration of NaClO dissolved large amounts of gelatin gel. When the interaction was terminated, pH of NaClO had changed to neutral. The organic tissue is dissolved under alkali conditions. However, we thought that there might be another factor involved, because the gelatin gel was dissolved under neutral conditions. The weight was concluded to have been lost by the generation of DMPO-X-like and hydroxyl radicals. We also interacted (Pro-Pro-Gly)10 and detected the changes in the structure using nuclear magnetic resonance. The triple helix structure was decreased and the random coil structure was increased by the hydrogen bond dissolution induced using NaClO.
Our findings suggest that the free radicals generated from NaClO finally result in the decomposition of organic tissue, as evidenced by its loss of weight.
