2017 Volume 8 Issue 2 Pages 110-114
Six-week-old male Wistar rats were placed under general anesthesia and rubber dam isolation technique was applied using a custom-made rubber dam clamp. Under a dental microscope, pulpectomy of the mesial root of the bilateral maxillary first molars was performed and the root canal was prepared with a Ni-Ti rotary file, after which the root canal was filled. Histological observations were made after 4 weeks. The results showed that root canal treatment can be effectively carried out in a microenvironment with no procedural accidents, and the safety and usefulness of medical treatment under a dental microscope was confirmed. Here we report the operative procedure.