Evaluation of the reagent from the LATEX H-FABP KIT (YAMASA) for the quantitative measurement of H-FABP

Abstract

Heart-type fatty acid-binding protein (H-FABP) is an early marker of acute coronary syndrome (ACS). Immunochromatography is a widely used method for the measurement of H-FABP. However, this method is restricted by individual differences owing to visual judgment, and only a qualitative judgment is possible. Therefore, we evaluated the reagent from the LATEX H-FABP KIT (YAMASA) for the quantitative measurement of H-FABP. This reagent had a favorable within-run precision coefficient of variation (CV) in the range of 0.65–3.98% and between-day precision CV in the range of 1.28–5.57%. Additionally, coexisting materials had no effect on the reaction. In ACS patients, the sensitivity and specificity were 72% and 59%, respectively. However, it was considered desirable to consider the time for obtaining a blood sample from a heart attack victim.