2016 Volume 65 Issue 5 Pages 565-569
Mean corpuscular volume (MCV) is used as an index to confirm the misidentification of patients because the volume varies slightly in each patient, compared with other erythrocyte indices. In this study, we examined how MCV is affected when a smaller amount of blood than the defined amount was collected in an EDTA-2K-added 3-mL blood container, using XN-9000. We measured hematological values in 5 healthy volunteers, using the tubes for 3-mL blood collection, to which 6-level amounts of blood from 0.3 to 4.5 mL were collected individually, and those to which 6-level amounts of EDTA-2K were added so that the same concentration per milliliter of blood was ensured. As a result, it was confirmed that MCV tended to be higher as EDTA-2K concentrations increased in all the samples. In addition, the plasma osmolarity measured when the blood was centrifuged after the measurement of hematological values became higher with the increase in EDTA-2K concentrations. On the basis of these findings, it was suggested that the increase in EDTA-2K concentrations and plasma osmolarity might affect MCV when the amount of collected blood was small. Since an EDTA-2K-added 3-mL blood container has a larger amount of EDTA-2K than a 2-mL container, it was estimated that MCV might be easily affected by the amount of collected blood.
