2016 Volume 65 Issue 5 Pages 557-564
The measurement of specific IgE in sera is useful for allergy diagnosis. MAST Immunosystems III (MAST III) is used for the screening of sensitized allergens in patients suspected of having allergy. However, the results of MASTIII do not agree with those of ImmunoCAP, which is used for further investigation. Recently, ViewAllergy has been developed as a multiple-allergen-specific IgE antibody measurement kit by Thermo Fisher Diagnostics K.K., which also manufactures ImmunoCAP. We assessed the basic characteristics of MAST III and ViewAllergy in order to compare their basic performances. From the results of the reproducibility test, the limit of the quantitation test, and the IgE interference study, ViewAllergy showed superior performance to MAST III. The positive concordance rate between these 2 kits for food allergens was low (less than 50%). These results were presumed to be caused by differences in allergen quality, measurement principle, and reproducibility of reagents. In conclusion, ViewAllergy demonstrated sufficient basic performance with superior accuracy as a screening test kit for multiple-allergen-specific IgE antibodies.
