2018 Volume 67 Issue 1 Pages 37-43
The latex turbidimetric immunoassay (LTIA) has been used for the measurement of matrix metalloproteinase (MMP)-3 levels. However, in the cases of healthy infants, the levels of serum MMP-3 are too low to detect by the LTIA method. In this study, we evaluated the analytical performance of a newly developed chemiluminescent enzyme immunoassay (CLEIA) kit, and compared the frequency of nonspecific reactions between the CLEIA and LTIA kits. The within-run and between-run coefficients of variation (CVs) for the newly developed CLEIA kit ranged from 1.1% to 1.6% and from 1.1% to 1.3%, respectively. The detection limit and quantitation limit (at 10% CV) of this new kit were 0.041 and 0.092 ng/mL, respectively. The assay results for the serially diluted sample of MMP-3 showed excellent linearity up to 2,575 ng/mL. The % recovery in the spiked recovery test was in the range from 99% to 107%. An interference test was performed, but no significant interference was observed. Moreover, there was a good correlation between the CLEIA kit and the two LTIA kits (r = 0.995 and r = 0.989). The serum MMP-3 levels were measured in 336 samples with various immunoserological abnormalities using the CLEIA kit and the two LTIA kits. There were 10 samples that showed value divergence among the 3 kits owing to a nonspecific reaction, but there was no sample that showed poor dilution linearity in measurements using the CLEIA kit. On the basis of these results, this newly developed CLEIA kit is useful as a measurement reagent for MMP-3.
