Abstract
A QIAGEN method using a QIAamp DNA Mini Kit, which is conventionally used to extract DNA, is complicated and takes a long time to perform. If we select the method using a Loopamp PURE DNA Extraction Kit (PURE), which is easier and faster than the QIAGEN method to perform, we expect to improve the efficiency of our work. We compared these methods. We investigated and compared the time necessary to extract DNA using a pharyngeal swab between the QIAGEN and PURE methods. Using the DNA extracted by these methods, we measured it with the Loopamp Mycoplasma Pneumoniae Detection Reagent Kit (LAMP), and compared the positive conformity ratio, negative conformity ratio, and overall conformity ratio obtained using LAMP with those obtained using the QIAGEN and PURE methods. This investigation is based on possible cases of mycoplasma in 27 pediatric patients. The results showed that 14 patients were positive, 13 patients were negative, and the overall conformity ratio was 100%. In addition, it was more time-efficient to extract DNA by the PURE method than by the QIAGEN method. In conclusion, using the PURE method, which is a new DNA extraction method, allows us to report results to the clinical department immediately and helps in treatments by facilitating diagnosis and judgments of treatment in pediatrics.
