2018 Volume 67 Issue 4 Pages 475-481
Fibrinogen, a major plasma protein coagulation factor, is a classic positive acute-phase reactant protein and is a risk factor for DIC and hemorrhage. Therefore, fibrinogen plays a key role in hemostasis and thrombosis. The coagulation cascade reaction in the blood coagulation test occurs upon mixing of the patient’s plasma and reagents, as fibrinogen cleaved by thrombin continuously polymerizes to form a fibrin polymer, and its molecular weight increases sequentially. In light scattering, the scattered light intensity changes as the molecular weight and particle diameter increase; thus, the coagulation reaction can be measured on the basis of light scattered intensity. The final scattered light intensity of this coagulation reaction represents the fibrinogen concentration. This method is used for the measurement of the fibrinogen concentration as a PT-driven method. However, the absorbance near the end of the coagulation increases slowly, and fluctuates. Therefore, the exact intensity at the end of coagulation is difficult to measure. As the coagulation reaction profile of CP3000 can be approximated from Gompertz growth curves, we estimated fibrin concentration from the maximum of this equation using a mathematical method. Comparison of the fibrinogen concentration obtained by the Clauss method with the maximum value of each item (PT and APTT) shows that the correlation coefficient between the Clauss method and each item obtained by this method was 0.962 or more. We considered that fibrinogen concentration can be estimated by this technique.
