2018 Volume 67 Issue 4 Pages 575-579
The patient, a woman in her forties with a family history of diabetes, was diagnosed by a previous physician as having diabetes and was admitted to our hospital for treatment. Her HbA1c level determined by the latex agglutination method as shown in her letter of introduction was 10.0%, which deviated significantly from the 5.5% obtained by the HPLC-723G8 (Tosoh) method in our hospital. The measurement principle of the G8 method is ion-exchange HPLC. Therefore, we were asked to measure HbA1c by ion-exchange HPLC precision analysis and affinity HPLC. An abnormal peak suggesting an abnormal hemoglobin level was observed in the ion-exchange HPLC precision analysis results, and the HbA1c level obtained by affinity HPLC was 8.8%. In the β-globin gene sequencing at Fukuyama Medical Laboratory, [Codon 22 GAA(Glu)→AAA(Lys)](heterozygote) HbE-Saskatoon was detected. We have to work on sharing this information with other medical staff members to understand the results of the specific analysis method.
