2018 Volume 67 Issue 4 Pages 519-523
The BD SurePathTM as a liquid-based cytology system has three types of preservative solution for cellular fixations (gyne for gynecologic materials, and red and blue for nongynecologic materials). Differences in their capability for antigenic preservation are expected in immunostaining because the three preservative solutions have different ingredients. The aim of this study was to determine the capability of each preservative solution for sequential antigenic preservation. Cellular sediments of pleural effusion from four cases of lung adenocarcinoma were stored for four different periods (1 hour, 1 week, 1 month, and 3 months). After each period, immunostaining of four markers (Ki67, p53, Cyclin A, and MCM7) was performed, and positivity rate was calculated. From the results, the following became obvious: (1) No consistent correlation was recognized in each marker with each preservative solution, although a decrease in positivity rate was recognized after each period. (2) As for the capability for antigenic preservation, no clear difference was recognized. (3) Because a decrease in positivity rate of 30% was found in a period of one-week preservation with an antibody, the possibility of incorrect decision on positivity was suggested. (4) After this, additional examination of cellular preservative methods using many types of markers that are used is necessary, and the development of an original immunostaining protocol is desired.
