Japanese Journal of Medical Technology
Online ISSN : 2188-5346
Print ISSN : 0915-8669
ISSN-L : 0915-8669
Materials
Basic performance evaluation of the automatic blood cell washing system “cell washer UltraCW II”: Usefulness in tests of blood transfusion
Kento NISHIDAChiaki KATOSatoru YOKOYAMAMoe KAWAKAMIHiroko ENDOTomomi WATANABETadashi MATSUSHITA
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2020 Volume 69 Issue 1 Pages 82-88

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Abstract

The basic requirements demanded from a red blood cell washing system in tests of blood transfusion are the efficiency of washing cells and a sufficient number of red blood cells remaining in a test tube. We tried to examine the usefulness of the automatic blood cell washing system called “cell washer UltraCW II” (Helmer) in a test of blood transfusion wherein the conditions were set for each parameter in the washing process. We calculated the amount of liquid that remained in the test tube from the difference in the weight of the test tube before and after washing cells. We calculated the washing dilution factor by measuring the absorbance at 478 nm using OrangeG. We calculated the number of red blood cells that remained in the hemolysis liquid with distilled water by measuring the absorbance at 541 nm. The factor that affected most the washing efficiency of red blood cells was the number of revolutions of DECANT. It made the red blood cells decrease in number when the number of washings (FILL) was too high. Extending the time of SPIN led to the extension of the washing time. Using a parameter provided by the manufacturer, we determined the intra-assay precisions of 24 test tubes. The washing dilution factor showed a mean of 109 and a CV of 3.7% for a 10 mm test tube, and a mean of 165 and a CV of 6.1% for a 12 mm test tube. The red blood cell residual rate showed a mean of 72.2% and a CV of 12.2% for a 10 mm test tube, and a mean of 78.6% and a CV of 5.2% for a 12 mm test tube. The results indicated a good performance of the system. In the anti-D titration with PEG-IAT in high-protein and high-globulin specimens, antiglobulins were not neutralized owing to washing insufficiency, and agglutination detection was possible. From these results, we found that UltraCWII was useful for the washing of red blood cells in the test of blood transfusion.

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© 2020 Japanese Association of Medical Technologists
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