2020 Volume 69 Issue 1 Pages 112-116
A female patient in her 80s was transported to our hospital with fever and hypoxia. Gram-negative bacillus cells were isolated from one aerobic blood culture vial. However, the bacterium was not identified by conventional phenotypic assays and matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry. A 16S ribosomal RNA (rRNA) gene sequencing technique revealed the bacterium to be Paenibacillus vini. P. vini has hardly been reported to be isolated from blood culture. In this study, we identified P. vini isolated from blood culture by the 16S rRNA gene sequencing technique. For the identification of Paenibacillus species, sequencing of the 16S rRNA gene was effective. In Spain, of the 136 Paenibacillus strains isolated between 1999 and 2015, only one strain was identified as P. vini.